During the process, Eugenol is extracted from the distillate with dichloromethane and analyzed using NMR spectrometers. As with many other volatile oils, clove oil inhibits gram-positive and gram-negative bacteria.
Essential oils are extracted, depending on the nature of the botanical material, by a variety of techniques, including expression cold-presssolvent, enfleurage cold-fat and supercritical fluid SCF extraction, and distillation.
Extraction of Eugenol from Cloves lesson plan. You can access and download the Steam distillation clove oil plan here: The bud also contains a tannin complex, a gum and resin, and a number of glucosides of sterols.
The aqueous layer can then be siphoned off using a separatory funnel. Explore our online Chemistry in the Classrooms to find additional lesson plans, videos and products that help empower students and teachers through instrumentation and resources.
If an emulsion develops, as is often the case in small-scale distillation, the immiscibility of the hydrophobic oil makes extraction and isolation of the product with a non-polar organic solvent highly effective.
Eugenol, the main ingredient in clove oil, is a familiar fragrance in many dental offices as it is often mixed into a paste and used in dentistry as a local antiseptic and anesthetic.
Since the oils are immiscible in water a two-phase distillate is produced, a water layer and an oil layer. The challenge for the students involved in the clove experiment is to extract sufficient quantities of eugenol. Steam vaporizes the high-boiling essential oils and the hot vapors condense back into a liquid, along with water, as they pass through a cooling system.
We have developed a lesson plan for chemistry teachers to conduct an experiment involving the extraction of eugenol from whole cloves by co-distillation with indirect steam, steam that is generated in situ. NMR spectroscopy provides a wealth of information on physical, electronic, chemical and structural aspects of molecular systems.
Collecting at least 60 mL of distillate can yield up to 2 mL of eugenol after extraction and evaporating off dichloromethane. Steam distillation is a co-distillation technique that uses live steam to separate components of a mixture.
The instrument procedure for sample analysis using an NMR spectrometer includes: Other constituents include flavonoids, carbohydrates, lipids, oleanolic acid, rhamnetin, and vitamins…. The oil is usually less dense and floats atop the water. It is a pale yellow oil with a warm, pungent, yet pleasing aroma, the smell of bay leaves and clove.
The experiment, which is moderately Steam distillation clove oil, takes about five hours to do the procedures and analysis.
Most essential oils are extracted by direct or indirect steam distillation. NMR spectroscopy is the method of choice for many organic chemists because of its versatility in elucidating molecular structure, optimizing reaction dynamics, measuring reaction kinetics, monitoring reaction content and controlling product purity.
Eugenol is also found in bay leaves and allspice, and other botanical oil, but in lower concentrations. Eye protection should be worn at all times during the experiment and each wall outlet used must be equipped with a 3-pronged grounded outlet, with the ground being a noncurrent-carrying wire connected to earth ground at the main distribution box.
Cloves are also said to have a positive effect on healing stomach ulcers…. Indirect steam distillation is a technique used for generating steam in situ, where the water level is kept below the plant material.
As with many experiments, safety precautions should be taken. It is a nondestructive technique ideally suited for measuring and recovering small sample quantities. It allows distillation to be performed at temperatures below the boiling points of the individual components.
Clove oil is reported to have antihistaminic and antispasmotic properties, most likely due to the presence of eugenyl acetate.Isolation of Eugenol from Cloves using steam distillation lab Jesus Perez-Sanchez 11/3/14 Chem lab, section: TA: Qi tong Abstract: The purpose of this experiment was to use techniques to do some isolation of natural products like solid cloves.
These techniques will include task that involve. Steam distillation is one way to isolate an essential oil from a plant or fruit. Describe two other methods.
Expression (or cold-pressing) is the process of mechanically squeezing the oils out of the source. Transcript of Experiment 1: Isolation of Eugenol from Cloves. Experiment 1: Steam Distillation Introduction Steam distillation and extraction were used to isolate eugenol from cloves Steam Distillation of Clove Oil presented by: Brittany Gentry Valerie Ng Nayeli Venegas.
Sep 12, · AROMATIC OIL STEAM DISTILLATION PLANT - Duration: How To Make Clove Oil at Home - SIMPLY & EASILY - Duration: Natural Health WISI Essential Oil Distillation Process. The experiment consists of three steps, steam distillation of essential oils from ground clove, extraction of oils from distillate, and identification of oil using GC and GC-MS.
Of these techniques, steam distillation is the most notable.5/5(1). Title: Experiment 4: Steam Distillation of a Volatile Oil from Cloves Purpose: To purify eugenol from clove leaves, analyze purity with GC, and obtain a percent yield.
Compo und Boiling point Hazards Hexane 69 C Highly Flamma ble, Harmful, Dangero us for environ ment Eugenol C Harmful Pre-lab: What is the theory behind steam distillation (i.e.
how does it work and how can it be used to.Download